Antigen

Antigen Class

TFs and others

Antigen

AFF1

Cell type

Cell type Class

Digestive tract

Cell type

DLD-1

Primary Tissue

Colon

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

DLD-1

cell line

Colorectal adenocarcinoma DLD-1

genotype

AFF1-AID OsTIR1

chip antibody

AF4 Bethyl A302-345A

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, 20-50 million cells were crosslinked with 1% paraformaldehyde (Electron Microscopy Sciences) in PBS for 10 min at r.t. Chromatin was sonicated with the Covaris E220 for 4 min with 10% duty cycle, 140 peak intensity power, 200 cycles per burst. Immunoprecipitations were carried out with antibodies and Protein A/G-agarose beads (Santa Cruz) or Dynabeads Protein G (Invitrogen). For ChIP-seq, DNA libraries were prepared by the HTP Library Preparation Kit for Illimina (KAPA) and sequenced on the NextSeq 500 or NovaSeq 6000 (Illumina).

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

25886712

Reads aligned (%)

93.6

Duplicates removed (%)

5.3

Number of peaks

791 (qval < 1E-05)

hg19

Number of total reads

25886712

Reads aligned (%)

92.9

Duplicates removed (%)

6.3

Number of peaks

945 (qval < 1E-05)